The Effect of Resistance Training with Moderate and Progressive Intensity on Indices of Oxidative Stress (Serum 8-Isoprostane, Malondialdehyde and Reduced Glutathione Levels) in Young Healthy Men

Ali Zabet 1 , Farshad Ghazalian 1 , * and Hojjatolah Nik-Bakht 1
Department of Physical Education, Science and Research Branch, Islamic Azad University, Tehran, Iran

Journal of Clinical Research in Paramedical Sciences

Background: 8-isoprostane(8-IsoP) and malondialdehyde (MDA) are novel and classic index of lipid per oxidation, respectively, and reduced glutathione (GSH) is an index of antioxidant status, which the chronic adaptation of moderate and progressive resistance training on them is unclear. The purpose of this study is to investigate and compare the effect of 9 weeks moderate and progressive resistance training on serum 8-isoprostane, malondialdehyde and reduced glutathione levels in young men (from 17- to 21 year-olds).

Methods: In the semi-experimental study, 36 volunteers were randomly divided in to 3 groups of 12 men involving: moderate and progressive resistance training and control groups. The training protocol involved three periods of three weeks each with progressive intensity. The moderate training group performed the first period of protocol in throughout of study (just 5 percent increase in training intensity as overload), while the training load of progressive training group was incrementally increased in every period. Blood sampling were taken in two phases involving pre test and following ninth week from anterior brachial vein. The variables were measured via sandwich ELISA.

Results: The results of ANCOVA and Bonferroni tests showed that 9 weeks of moderate and progressive resistance training led to significance decrease in lipid oxidation indices (8-IsoP and MDA);the range of the decline especially in serum 8-IsoP was more in progressive group. Significant change in serum GSH levels was not observed in the training groups.

Conclusions: The results of this study show that periodic increase in resistance training load leads to improves in oxidative stress adaptability. Moreover, 8-IsoP is more accurate index for predicting oxidative stress compared to MDA.

Keywords: Resistance Training; Oxidative Stress; Glutathione; 8-Isoprostane; Malondialdehyde

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