The effect of 8 weeks of interval training combined with the consumption of cineole, linalool, and bourbonene on MAPK/Arc gene expression and learning in rats with Alzheimer’s disease-Exercise physiology

The effect of 8 weeks of interval training combined with the consumption of cineole, linalool, and bourbonene on MAPK/Arc gene expression and learning in rats with Alzheimer’s disease-Exercise physiology

Iranepf

J Shahrekord Univ Med Sci

Elham Majidi 1 ORCID logo, Khosro Jalali Dehkordi 1* ORCID logo, Farzaneh Taghian 1 ORCID logo, Seyed Ali Hosseini 2 ORCID logo

۱ Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
۲ Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

Abstract

Background and aims: This research aimed to investigate the effect of 8 weeks of interval training and the use of cineole, linalool, and bourbonene on MAPK/Arc gene expression and learning in Alzheimer’s disease (AD) rats.

Methods: In this experimental study, 40 AD rats were randomized into eight groups, including (1) control, (2) AD, (3) AD+aerobic training (AT), (4) AD+linalool, cineole, and bourbonene (LCB), (5) AD+AT+LCB, (6) AT+AD, (7) LCB+AD, and (8) AT+LCB+AD. AD was induced by injecting amyloid-beta (Aβ۱)-۴۲ into the hippocampus of rats. The interval training protocol was performed five days per week for eight weeks before and after AD induction. Linalool at a concentration of 25 mg/kg, cineole at a concentration of 10 µM, and β-bourbonene at a concentration of 10 µg/mL were used for eight weeks. One-way analysis of variance was used for between-group comparisons, and Tukey’s test was used for pairwise comparisons at P≤۰٫۰۵٫

Results: AD induction caused a significant decrease in MAPK/Arc gene expression in hippocampal tissue (P=0.001). Interval exercise and consumption of three herbal drugs significantly increased gene expression of Arc (P=0.001) and MAPK (P=0.001). AD induction decreased learning (P=0.001). Interval exercise and consumption of three herbal medicines caused a significant increase in learning (P=0.001).

Conclusion: Interval exercise and using three herbal medicines have more favorable effects on improving MAPK/Arc gene expression and learning in AD than each alone.
Keywords: Interval training, Cineole, Linalool, Bourbonene, MAPK, Arc, Learning, Alzheimer’s disease, Exercise physiology

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