Cardioprotective Effect of High-Intensity Aerobic Interval Training against Adriamycin-Induced Cardiac Toxicity in Rats

Khadijeh Ebrahimi 1 , * , Siroos Choobineh 2 , ** , Rahman Soori 2 and Reza Badalzadeh 3
Authors Information
۱ Department of Physical Education and Sport Sciences, Marand Branch, Islamic Azad University, Marand, Iran
۲ Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
۳ Molecular Medicine Research Center and Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Journal of Clinical Research in Paramedical Sciences

Abstract

Background: Adriamycin (ADR) is a useful drug for the treatment of hematologic malignancies and solid tumors. However, its clinical uses are limited due to dose-dependent cardiac toxicity. Contrary to moderate endurance training, there is little research about the protective role of high-intensity aerobic interval training (HIIT) against the ADR-induced cardiac toxicity and its mechanisms.

Objectives: The present study aimed to investigate the protective effect of HIIT against ADR-induced cardiac toxicity in the left ventricle of rats by assessment of the serum biomarkers of cardiac injury (LDH and CK-MB).

Methods: Twenty-four male Wistar rats were randomly assigned into four groups (n = 6/group): (1) Control; (2) ADR (20 mg/kg.bw); 3) HIIT (7 sets of 4 minutes intervals at 80% – 90% VO2max interspersed with 3 minutes periods of 65% – 75% VO2max, for 8 weeks), and (4) HIIT + ADR. The ELISA method was used for measuring the serum levels of biomarkers. Statistical differences were analyzed using one-way analysis of variance followed by Tukey’s post hoc test for multiple comparisons (α < 0.05).

Results: Based on the results, ADR-induction resulted in a significant increase in the LDH and CK-MB levels compared to the ADR group (P < 0.05). Also, the HIIT per se insignificantly increased the levels of LDH and CK-MB compared to the control group. However, the HIIT before ADR-induction resulted in a significant decrease in the LDH and CK-MB levels compared to the ADR group (P < 0.05).

Conclusions: Therefore, the HIIT can be a proper non-prescriptive strategy for preventing ADR-induced cardiotoxicity via reducing the serum biomarkers of cardiac injury.

Keywords: Interval-Training; Adriamycin; Cardiac Toxicity; Cardiac Biomarkers

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