Yaser Alavi; Shadmehr Mirdar; Mohamad Rostamani
Iranian Journal of Health and Physical activity
Purpose: The aim of the current research was to assess effects of caffeine (5 and 9 mg.kg -1 ) ingestions concomitant to incremental running test on malondialdehyde (MDA) as lipid peroxidation marker and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) as enzymatic antioxidants.
Material and Methods: Ten subjects were tested on a treadmill until exhaustion on three separate occasions between which there were intervals of 5 days. Every volunteer underwent three conditions containing placebo or caffeine doses that were consumed one hour before exercise (pre-test). Blood samples were collected pre-test, before caffeine or placebo consumption and immediately after exercise (post-test).
Results: Results showed that in comparison to placebo condition, caffeine doses decreased MDA levels and increased GPx activities significantly (P< 0/05). Regardless of 5 mg.kg -1 caffeine ingestion that had no significant effect on CAT and SOD activities (P< 0/05), 9 mg.kg -1 caffeine intake significantly increased and decreased CAT and SOD respectively (P< 0/05), when compared to placebo. For MDA levels, there was no significant difference between caffeine doses (P< 0/05), but these differences for CAT, SOD and GPx were significant (P< 0/05).
Discussion and Conclusion: In conclusion caffeine doses approximately had equal effects on lipid peroxidation decline, while these effects on enzymatic antioxidant with 9 mg.kg -1 caffeine intake were better than 5 mg.kg-1 caffeine.
Oxidative stress; Free radicals; Progressive exercise; Bruce protoco; Caffeine doses